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Subject: =?utf-8?Q?Webinar=3A=20Unraveling=20the=20Potential=20of=20Neural=20Stem=20Cells=3A=20Antibodies=20as=20Key=20Tools?=
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Date: Tue, 18 Jul 2023 04:04:18 +0000
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** Unraveling the Potential of Neural Stem Cells: Antibodies as Key Tools
------------------------------------------------------------
C.E. Credits: P.A.C.E. CE =EF=BD=9C Florida CE (https://www.labroots.com/m=
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Jamuna KS
Scientist II=2C Cell Biology=2C Thermo Fisher Scientific

Abstract
Stem cells have the capability to develop into any specialized cell type=
=2C which makes them a valuable resource in research and regenerative medi=
cine. Differentiated stem cell models provide insights into proteins invol=
ved in disease/developmental processes=2C which would otherwise be inacces=
sible for research due to their rare tissue origin and ethical constraints=
=2E Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) d=
ifferentiated to various lineages serve as powerful tools to study the pro=
teins of interest specific to a cell type. Similarly=2C neural stem cells=
 (NSCs) have revolutionized the research on neuropsychiatry and neurodegen=
eration disorders=2C as they can be differentiated into neuronal subtypes=
 in vitro. In this seminar=2C we showcased the use of human episomal iPSCs=
 and NSCs to generate physiologically relevant neural models such as retin=
al ganglion cells=2C neuroepithelial cells=2C neural rosettes=2C and astro=
cytes. We have also differentiated iPSCs into dopaminergic
neurons which help in studying the underlying mechanisms of Parkinson's di=
sease. These studies are important for deciphering the cellular and molecu=
lar mechanisms underlying neurogenesis for optimizing stem cell-based trea=
tment of neurological disorders and injuries.

Learning Objectives
* Outline the overview of pluripotent stem cells and neural stem cells
* Explain the differentiations into committed neural cell types
* Identify the characterization of the differentiated models using antibod=
ies
* Generate disease model for Parkinson's disease

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dyContainer" style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust:=
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0" cellspacing=3D"0" width=3D"100%" class=3D"mcnDividerBlock" style=3D"min=
-width: 100%;border-collapse: collapse;mso-table-lspace: 0pt;mso-table-rsp=
ace: 0pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;table-l=
ayout: fixed !important;">
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dding: 10px 18px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;=
-webkit-text-size-adjust: 100%;">
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g=3D"0" cellspacing=3D"0" width=3D"100%" style=3D"min-width: 100%;border-t=
op: 2px none #EAEAEA;border-collapse: collapse;mso-table-lspace: 0pt;mso-t=
able-rspace: 0pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%=
;">
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t-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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</table><table border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" class=3D"mcnTextBlock" style=3D"min-width: 100%;border-collapse: coll=
apse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 100=
%;-webkit-text-size-adjust: 100%;">
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        <tr>
            <td valign=3D"top" class=3D"mcnTextBlockInner" style=3D"paddin=
g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
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<h1 style=3D"text-align: left;display: block;m=
argin: 0;padding: 0;color: #535353;font-family: 'Open Sans'=2C 'Helvetica=
 Neue'=2C Helvetica=2C Arial=2C sans-serif;font-size: 26px;font-style: nor=
mal;font-weight: bold;line-height: 125%;letter-spacing: normal;"><span sty=
le=3D"font-size:24px">Unraveling the Potential of Neural Stem Cells: Antib=
odies as Key Tools</span></h1>
<br>
C.E. Credits:<strong><u>&nbsp;<a href=3D"https://labroots.us7.list-manage.=
com/track/click?u=3D6f39393ef38fb324428ff8c2c&id=3Dd78a07e5ff&e=3De3678a3621" target=3D"_blank" style=3D"mso-line-height-rule: exactly;-ms-text-siz=
e-adjust: 100%;-webkit-text-size-adjust: 100%;color: #009cde;font-weight:=
 normal;text-decoration: underline;"><span style=3D"color:#E71715">P.A.C.E=
=2E CE&nbsp;=EF=BD=9C&nbsp;Florida CE</span></a></u></strong>
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=09=09=09=09

<span style=3D"font-size:15px"><span style=3D"=
font-family:arial=2Chelvetica neue=2Chelvetica=2Csans-serif"><em><strong><=
a href=3D"https://labroots.us7.list-manage.com/track/click?u=3D6f39393ef38=
fb324428ff8c2c&id=3D662946900e&e=3De3678a3621" target=3D"_blank" style=3D=
"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text-siz=
e-adjust: 100%;color: #009cde;font-weight: normal;text-decoration: underli=
ne;"><img data-file-id=3D"42943" height=3D"12" src=3D"https://gallery.mail=
chimp.com/6f39393ef38fb324428ff8c2c/images/b9d0b3b5-66ac-4b86-857f-08e5d0e=
67a46.png" style=3D"border: 0px;width: 20px;height: 12px;margin: 0px;outli=
ne: none;text-decoration: none;-ms-interpolation-mode: bicubic;" width=3D"=
20"></a></strong></em><strong><span style=3D"color:#E71715">August 02=2C 2=
023&nbsp;</span></strong><em><strong>|</strong></em> </span></span><span s=
tyle=3D"font-size:14px"><span style=3D"font-family:arial=2Chelvetica neue=
=2Chelvetica=2Csans-serif">10:30am (IST)=2C 1:00pm (SGT)=2C 2:00pm (KST)=
=2C 4:00pm (AEDT)=2C&nbsp; &nbsp; &nbsp; &nbsp; 6:00pm (NZDT)</span></span=
>
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=09=09=09=09

=09=09=09=09
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align: center;text-decoration: none;color: #FFFFFF;mso-line-height-rule: e=
xactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;display:=
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epeat: no-repeat;background-position: center;background-size: cover;border=
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t-size-adjust: 100%;-webkit-text-size-adjust: 100%;max-width: 600px !impor=
tant;">
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o-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-a=
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er=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"300" class=3D"column=
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e-adjust: 100%;background-color: #ffffff;background-image: none;background=
-repeat: no-repeat;background-position: center;background-size: cover;bord=
er-top: 0;border-bottom: 2px solid #EAEAEA;padding-top: 0;padding-bottom:=
 9px;">
                                    
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ellpadding=3D"0" cellspacing=3D"0" width=3D"100%" class=3D"templateContain=
er" style=3D"border-collapse: collapse;mso-table-lspace: 0pt;mso-table-rsp=
ace: 0pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;max-wid=
th: 600px !important;">
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dyContainer" style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust:=
 100%;-webkit-text-size-adjust: 100%;"><table border=3D"0" cellpadding=3D"=
0" cellspacing=3D"0" width=3D"100%" class=3D"mcnTextBlock" style=3D"min-wi=
dth: 100%;border-collapse: collapse;mso-table-lspace: 0pt;mso-table-rspace=
: 0pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
                    <tbody><tr>

<span style=3D"font-size:16px"><span style=3D"=
font-family:arial=2Chelvetica neue=2Chelvetica=2Csans-serif"><strong>Prese=
nted By:</strong></span></span>
                        </td>
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                </tbody></table>
=09=09=09=09

</td>
                </tr>
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            <table class=3D"mcnCaptionRightTextContentContainer" align=3D"=
right" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"396" styl=
e=3D"border-collapse: collapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt=
;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
                <tbody><tr>
                    <td valign=3D"top" class=3D"mcnTextContent" style=3D"m=
so-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-=
adjust: 100%;word-break: break-word;color: #535353;font-family: Helvetica;=
font-size: 16px;line-height: 150%;text-align: left;">
                        <div style=3D"text-align: left;"><span style=3D"fo=
nt-size:16px"><span style=3D"font-family:arial=2Chelvetica neue=2Chelvetic=
a=2Csans-serif"><span style=3D"color:#E71715"><strong>Jamuna KS</strong></=
span></span></span><br>
<span style=3D"font-size:14px"><span style=3D"font-family:arial=2Chelvetic=
a neue=2Chelvetica=2Csans-serif">Scientist II=2C Cell Biology=2C Thermo Fi=
sher Scientific</span></span></div>

</td>
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</tbody></table>

</td>
        </tr>
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</table><table border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" class=3D"mcnDividerBlock" style=3D"min-width: 100%;border-collapse: c=
ollapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust:=
 100%;-webkit-text-size-adjust: 100%;table-layout: fixed !important;">
    <tbody class=3D"mcnDividerBlockOuter">
        <tr>
            <td class=3D"mcnDividerBlockInner" style=3D"min-width: 100%;pa=
dding: 18px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webk=
it-text-size-adjust: 100%;">
                <table class=3D"mcnDividerContent" border=3D"0" cellpaddin=
g=3D"0" cellspacing=3D"0" width=3D"100%" style=3D"min-width: 100%;border-t=
op: 2px solid #EAEAEA;border-collapse: collapse;mso-table-lspace: 0pt;mso-=
table-rspace: 0pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100=
%;">
                    <tbody><tr>
                        <td style=3D"mso-line-height-rule: exactly;-ms-tex=
t-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
                            <span></span>
                        </td>
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                </tbody></table>

            </td>
        </tr>
    </tbody>
</table><table border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" class=3D"mcnTextBlock" style=3D"min-width: 100%;border-collapse: coll=
apse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 100=
%;-webkit-text-size-adjust: 100%;">
    <tbody class=3D"mcnTextBlockOuter">
        <tr>
            <td valign=3D"top" class=3D"mcnTextBlockInner" style=3D"paddin=
g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
                    <tbody><tr>

<p style=3D"margin: 10px 0;padding: 0;mso-line=
-height-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust:=
 100%;color: #535353;font-family: Helvetica;font-size: 16px;line-height: 1=
50%;text-align: left;"><span style=3D"font-size:14px"><span style=3D"font-=
family:arial=2Chelvetica neue=2Chelvetica=2Csans-serif"><strong>Abstract</=
strong><br>
Stem cells have the capability to develop into any specialized cell type=
=2C which makes them a valuable resource in research and regenerative medi=
cine. Differentiated stem cell models provide insights into proteins invol=
ved in disease/developmental processes=2C which would otherwise be inacces=
sible for research due to their rare tissue origin and ethical constraints=
=2E Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) d=
ifferentiated to various lineages serve as powerful tools to study the pro=
teins of interest specific to a cell type. Similarly=2C neural stem cells=
 (NSCs) have revolutionized the research on neuropsychiatry and neurodegen=
eration disorders=2C as they can be differentiated into neuronal subtypes=
 in vitro. In this seminar=2C we showcased the use of human episomal iPSCs=
 and NSCs to generate physiologically relevant neural models such as retin=
al ganglion cells=2C neuroepithelial cells=2C neural rosettes=2C and astro=
cytes. We have also differentiated iPSCs into dopaminergic neurons which h=
elp in studying the underlying mechanisms of Parkinson's disease. These st=
udies are important for deciphering the cellular and molecular mechanisms=
 underlying neurogenesis for optimizing stem cell-based treatment of neuro=
logical disorders and injuries.<br>
&nbsp;<br>
<strong>Learning Objectives</strong></span></span></p>

<ul>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;">Outline the overview of pluripotent stem c=
ells and neural stem cells</li>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;"><span style=3D"font-size:14px"><span style=
=3D"font-family:arial=2Chelvetica neue=2Chelvetica=2Csans-serif">Explain t=
he differentiations into committed neural cell types</span></span></li>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;"><span style=3D"font-size:14px"><span style=
=3D"font-family:arial=2Chelvetica neue=2Chelvetica=2Csans-serif">Identify=
 the characterization of the differentiated models using antibodies</span>=
</span></li>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;"><span style=3D"font-size:14px"><span style=
=3D"font-family:arial=2Chelvetica neue=2Chelvetica=2Csans-serif">Generate=
 disease model for Parkinson's disease</span></span></li>
</ul>

</td>
                    </tr>
                </tbody></table>
=09=09=09=09

<table align=3D"left" border=3D"0"=
 cellpadding=3D"0" cellspacing=3D"0" style=3D"border-collapse: collapse;ms=
o-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 100%;-webk=
it-text-size-adjust: 100%;">
                                            <tbody><tr>
                                                <td valign=3D"top" style=
=3D"padding-right: 9px;padding-bottom: 9px;mso-line-height-rule: exactly;-=
ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;" class=3D"mcnSha=
reContentItemContainer">
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llpadding=3D"0" cellspacing=3D"0" width=3D"" class=3D"mcnShareContentItem"=
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: 0pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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eft" valign=3D"middle" style=3D"padding-top: 5px;padding-right: 9px;paddin=
g-bottom: 5px;padding-left: 9px;mso-line-height-rule: exactly;-ms-text-siz=
e-adjust: 100%;-webkit-text-size-adjust: 100%;">
                                                                <table ali=
gn=3D"left" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"" st=
yle=3D"border-collapse: collapse;mso-table-lspace: 0pt;mso-table-rspace: 0=
pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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><tr>
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d align=3D"center" valign=3D"middle" width=3D"24" class=3D"mcnShareIconCon=
tent" style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-w=
ebkit-text-size-adjust: 100%;">
                                                                         =
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ef38fb324428ff8c2c&id=3D5286ebe8d4&e=3De3678a3621" target=3D"_blank" styl=
e=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text=
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