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Subject: =?utf-8?Q?Gibco=20Cell=20Culture=20Heroes=20=2D=C2=A0Investigating=20the=20biology=20of=20cell=2Din=2Dcell=20structures=20in=20the=20human=20liver=20using=20a=20range=20of=20microscopy=20modalities?=
From: =?utf-8?Q?Labroots=20for=20Thermo=20Fisher=20Scientific=2FGibco?= <info@labroots.com>
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Date: Tue, 25 Apr 2023 18:03:32 +0000
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Free Webinar - Register Today!

View this email in your browser (https://mailchi.mp/labroots/generating-pr=
otein-reagents-to-evaluate-the-antibody-and-cd8-t-cell-responses-to-sars-c=
ov-242310?e=3D93eddcbc4e)
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** Gibco Cell Culture Heroes - Scott Philip Davies=2C PhD - Investigating=
 the biology of cell-in-cell structures in the human liver using a range o=
f microscopy modalities
------------------------------------------------------------

C.E. Credits:https://www.labroots.com/ms/webinar/slc25a46-novel-mitochondr=
ial-regulator-biomarker-breast-cancer?campaign=3DlremailP.A.C.E. CE  (http=
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ail) =EF=BD=9C  (https://www.labroots.com/ms/webinar/gibco-cell-culture-he=
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-huma?campaign=3Dlremail) Florida CE (https://www.labroots.com/ms/webinar/=
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May 09=2C 2023
https://www.labroots.com/intercall/add-to-calendar/Webinar/8009?start-date=
=3D20230509T050000&end-date=3D20230509T060000&TZID=3DAmerica/Los_Angeles1:=
00pm BST=2C 7:00am EST=2C
6:00am (CST)
Presented by:
https://www.labroots.com/ms/webinar/gibco-cell-culture-heroes-scott-philip=
-davies-phd-investigating-biology-cell-in-cell-structures-huma?campaign=3D=
lremail
Scott Philip Davies=2C PhD=2C MRes=2C MSci
Postdoctoral Research Fellow=2C University of Birmingham=2C UK

=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
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=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D

Abstract
Join the Gibco Cell Culture Heroes webinar featuring Scott Philip Davies=
=2C PhD=2C MRes=2C MSci as he presents "Investigating the biology of cell-=
in-cell structures in the human liver using a range of microscopy modaliti=
es." Scott will share how advances in high content imaging and automated a=
nalysis have improved the understanding of lymphocyte cell-in-cell structu=
res formation in the liver.

Advances in high content imaging (HCI) have improved the use of cell cultu=
re to make a large number of comparisons within individual experiments. Th=
ese platforms reduce the number of cells needed to make these comparisons=
 and also allow assessments to be made over time. This is poignant when in=
vestigating poorly understood roles and mechanisms of dynamic cellular int=
eractions. These include processes which generate cell-in-cell structures=
 (CICS)=2C which describes the presence of one cell inside another. Many n=
ew forms of CICS have been described within the human liver in recent year=
s=2C which mostly involve the entry of a lymphocyte into epithelial and pa=
renchymal cells.  In this talk=2C I will outline my experience of using HC=
I and automated analysis to understand lymphocyte CICS formation in the li=
ver. I will also focus on how we can combine observations made in vivo usi=
ng fluorescence-based immunohistochemistry to interpret and plan efficient=
 HCI assays for understanding the formation and
consequences of CICS. I will also show how results made from 2D cell cultu=
re systems can be validated ex vivo using multiphoton microscopy.

Learning Objectives
* Outline the benefits of combining high content imaging with automated an=
alysis
* Discuss the biology of cell-in-cell structures in the human liver and th=
eir roles in disease
* Demonstrate the power of confirming observations in vitro=2C in vivo and=
 ex vivo using different and appropriate forms of microscopy

** Register now (https://www.labroots.com/ms/webinar/gibco-cell-culture-he=
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              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
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<h1 style=3D"display: block;margin: 0;padding:=
 0;color: #333333;font-family: Helvetica;font-size: 24px;font-style: norma=
l;font-weight: normal;line-height: 125%;letter-spacing: normal;text-align:=
 left;">Gibco Cell Culture Heroes - Scott Philip Davies=2C PhD - Investiga=
ting the biology of cell-in-cell structures in the human liver using a ran=
ge of microscopy modalities</h1>

<p style=3D"text-align: left;margin: 10px 0;padding: 0;mso-line-height-rul=
e: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;color=
: #202020;font-family: Helvetica;font-size: 14px;line-height: 150%;">C.E.=
 Credits:<a href=3D"https://labroots.us7.list-manage.com/track/click?u=3D6=
f39393ef38fb324428ff8c2c&id=3D7de155f777&e=3D93eddcbc4e" target=3D"_blank=
" style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;color: #1e8ae7;font-weight: normal;text-decoratio=
n: underline;"> </a><a href=3D"https://labroots.us7.list-manage.com/track/=
click?u=3D6f39393ef38fb324428ff8c2c&id=3De0784e5b71&e=3D93eddcbc4e" targe=
t=3D"_blank" style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust:=
 100%;-webkit-text-size-adjust: 100%;color: #1e8ae7;font-weight: normal;te=
xt-decoration: underline;"><span style=3D"color:#01427A">P.A.C.E. CE&nbsp;=
</span></a><a href=3D"https://labroots.us7.list-manage.com/track/click?u=
=3D6f39393ef38fb324428ff8c2c&id=3Dd38801b078&e=3D93eddcbc4e" style=3D"mso=
-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-ad=
just: 100%;color: #1e8ae7;font-weight: normal;text-decoration: underline;"=
><span style=3D"color:#01427A">=EF=BD=9C&nbsp;</span></a><a href=3D"https:=
//labroots.us7.list-manage.com/track/click?u=3D6f39393ef38fb324428ff8c2c&i=
d=3Da7ec2b0c39&e=3D93eddcbc4e" style=3D"mso-line-height-rule: exactly;-ms=
-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;color: #1e8ae7;font=
-weight: normal;text-decoration: underline;"><span style=3D"color:#01427A"=
>Florida CE</span></a></p>

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=09=09=09=09

=09=09=09=09
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t;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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lumnContainer" style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust=
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idth: 100%;border-collapse: collapse;mso-table-lspace: 0pt;mso-table-rspac=
e: 0pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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              =09
=09=09=09
=09=09=09=09
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pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
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<strong>Presented by:</strong>
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=09=09=09=09

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00%" class=3D"mcnTextBlock" style=3D"min-width: 100%;border-collapse: coll=
apse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 100=
%;-webkit-text-size-adjust: 100%;">
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g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
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<span style=3D"font-size:13px"><span style=3D"=
color:#01427A"><strong>Scott Philip Davies=2C PhD=2C MRes=2C MSci</strong>=
</span><br>
Postdoctoral Research Fellow=2C University of Birmingham=2C UK</span>
                        </td>
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=09=09=09=09

=09=09=09=09
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tyle=3D"background:#ffffff none no-repeat center/cover;mso-line-height-rul=
e: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;backg=
round-color: #ffffff;background-image: none;background-repeat: no-repeat;b=
ackground-position: center;background-size: cover;border-top: 0;border-bot=
tom: 0;padding-top: 9px;padding-bottom: 9px;"><table border=3D"0" cellpadd=
ing=3D"0" cellspacing=3D"0" width=3D"100%" class=3D"mcnTextBlock" style=3D=
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              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
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<p style=3D"font-size: 14px;text-align: left;m=
argin: 10px 0;padding: 0;mso-line-height-rule: exactly;-ms-text-size-adjus=
t: 100%;-webkit-text-size-adjust: 100%;color: #555555;font-family: Helveti=
ca;line-height: 150%;"><span style=3D"font-family:arial=2Chelvetica neue=
=2Chelvetica=2Csans-serif"><span style=3D"font-size:14px"><strong>Abstract=
</strong></span></span></p>
<span style=3D"font-family:arial=2Chelvetica neue=2Chelvetica=2Csans-serif=
"><span style=3D"font-size:14px">Join the Gibco Cell Culture Heroes webina=
r featuring Scott Philip Davies=2C PhD=2C MRes=2C MSci as he presents "Inv=
estigating the biology of cell-in-cell structures in the human liver using=
 a range of microscopy modalities." Scott will share how advances in high=
 content imaging and automated analysis have improved the understanding of=
 lymphocyte cell-in-cell structures formation in the liver.<br>
&nbsp;<br>
Advances in high content imaging (HCI) have improved the use of cell cultu=
re to make a large number of comparisons within individual experiments. Th=
ese platforms reduce the number of cells needed to make these comparisons=
 and also allow assessments to be made over time. This is poignant when in=
vestigating poorly understood roles and mechanisms of dynamic cellular int=
eractions. These include processes which generate cell-in-cell structures=
 (CICS)=2C which describes the presence of one cell inside another. Many n=
ew forms of CICS have been described within the human liver in recent year=
s=2C which mostly involve the entry of a lymphocyte into epithelial and pa=
renchymal cells. &nbsp;In this talk=2C I will outline my experience of usi=
ng HCI and automated analysis to understand lymphocyte CICS formation in t=
he liver. I will also focus on how we can combine observations made in viv=
o using fluorescence-based immunohistochemistry to interpret and plan effi=
cient HCI assays for understanding the formation and consequences of CICS.=
 I will also show how results made from 2D cell culture systems can be val=
idated ex vivo using multiphoton microscopy.<br>
<strong>&nbsp;<br>
Learning Objectives</strong></span></span>

<ul>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;"><span style=3D"font-family:arial=2Chelveti=
ca neue=2Chelvetica=2Csans-serif"><span style=3D"font-size:14px">Outline t=
he benefits of combining high content imaging with automated analysis</spa=
n></span></li>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;"><span style=3D"font-family:arial=2Chelveti=
ca neue=2Chelvetica=2Csans-serif"><span style=3D"font-size:14px">Discuss t=
he biology of cell-in-cell structures in the human liver and their roles i=
n disease</span></span></li>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;"><span style=3D"font-family:arial=2Chelveti=
ca neue=2Chelvetica=2Csans-serif"><span style=3D"font-size:14px">Demonstra=
te the power of confirming observations in vitro=2C in vivo and ex vivo us=
ing different and appropriate forms of microscopy</span></span></li>
</ul>

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=09=09=09=09

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                                                                         =
   <a href=3D"https://labroots.us7.list-manage.com/track/click?u=3D6f39393=
ef38fb324428ff8c2c&id=3D61a9749d64&e=3D93eddcbc4e" target=3D"_blank" styl=
e=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text=
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rpolation-mode: bicubic;" height=3D"24" width=3D"24" class=3D""></a>
                                                                        </=
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le: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">Tw=
eet</a>
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table>
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<table align=3D"left" border=3D"0"=
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o-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 100%;-webk=
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are</a>
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</td>
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</table><table border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" class=3D"mcnTextBlock" style=3D"min-width: 100%;border-collapse: coll=
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=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
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<p style=3D"font-size: 14px;text-align: center=
;margin: 10px 0;padding: 0;mso-line-height-rule: exactly;-ms-text-size-adj=
ust: 100%;-webkit-text-size-adjust: 100%;color: #555555;font-family: Helve=
tica;line-height: 150%;"><strong>Can't attend live?</strong> <a href=3D"ht=
tps://labroots.us7.list-manage.com/track/click?u=3D6f39393ef38fb324428ff8c=
2c&id=3D0590d2d67f&e=3D93eddcbc4e" target=3D"_blank" style=3D"mso-line-he=
ight-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 10=
0%;color: #1e8ae7;font-weight: normal;text-decoration: underline;"><span s=
tyle=3D"color:#01427A">Click here</span></a><a href=3D"https://labroots.us=
7.list-manage.com/track/click?u=3D6f39393ef38fb324428ff8c2c&id=3D1f51381dd=
f&e=3D93eddcbc4e" style=3D"mso-line-height-rule: exactly;-ms-text-size-ad=
just: 100%;-webkit-text-size-adjust: 100%;color: #1e8ae7;font-weight: norm=
al;text-decoration: underline;"> </a>to register and receive a link to the=
 recorded webcast.</p>
Labroots is approved as a provider of continuing education programs in the=
 clinical laboratory sciences by the ASCLS P.A.C.E. =C2=AE Program. By wat=
ching this webinar=2C you can earn 1 Continuing Education credit.
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=09=09=09=09

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00%" class=3D"mcnDividerBlock" style=3D"min-width: 100%;background-color:=
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