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Subject: =?utf-8?Q?Free=20Webinar=3A=20Considerations=20to=20Limit=20Off=2DTarget=20Edits=20in=20your=20Gene=20Editing=20Experiments?=
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Date: Tue, 21 Feb 2023 19:00:44 +0000
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Free Webinar - Register Today!

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** Considerations to Limit Off-Target Edits in your Gene Editing Experimen=
ts
------------------------------------------------------------
C.E. Credits: P.A.C.E. CE =EF=BD=9C Florida CE (https://www.labroots.com/m=
s/webinar/considerations-limit-off-target-edits-gene-editing-experiments?c=
ampaign=3Dlremail)

March 08=2C 2023
https://www.labroots.com/intercall/add-to-calendar/Webinar/7957?start-date=
=3D20230308T080000&end-date=3D20230308T090000&TZID=3DAmerica/Los_Angeles8:=
00am (PST)=2C 11:00am (EST)=2C
5:00pm (CET)
Presented by:
https://www.labroots.com/ms/webinar/considerations-limit-off-target-edits-=
gene-editing-experiments?campaign=3Dlremail
Kevin Pridham
Field Applications Scientist=2C
Thermo Fisher Scientific

=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
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=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D

Abstract
Engineered nucleases like the CRISPR-Cas9 system provide researchers with=
 powerful tools for the manipulation of the genome to create cell-based di=
sease models=2C animal models=2C and cell therapies for clinical implement=
ation. These editing modalities can achieve high editing efficiencies in a=
 wide range of different cell types and gene loci. However=2C this high ed=
iting efficiency comes at a price: Off target cleavage. Growing concerns f=
or off-target editing and the downstream results of such edits have resear=
chers looking for best practices in their genome editing experiments. In t=
his presentation=2C we will explore strategies to limit off-target edits i=
n your gene editing experiments=2C share considerations to take in differe=
nt experimental scenarios=2C and demonstrate the efforts of Thermo Fisher=
 Scientific to optimize the gene editing workflow - simplifying it for all=
 researchers.

Learning Objectives
* Summarize the impact of off-target edits in experiments
* Discuss the methods to detect=2C identify=2C and limit off-target edits=
 in your gene editing experiments
* Assess solutions to streamline and improve the gene editing workflow

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t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
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<h1 style=3D"display: block;margin: 0;padding:=
 0;color: #333333;font-family: Helvetica;font-size: 24px;font-style: norma=
l;font-weight: normal;line-height: 125%;letter-spacing: normal;text-align:=
 left;">Considerations to Limit Off-Target Edits in your Gene Editing Expe=
riments</h1>
<br>
C.E. Credits:&nbsp;<a href=3D"https://labroots.us7.list-manage.com/track/c=
lick?u=3D6f39393ef38fb324428ff8c2c&id=3D9afdf4ba1c&e=3D6670ee6f6b" target=
=3D"_blank" style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 1=
00%;-webkit-text-size-adjust: 100%;color: #1e8ae7;font-weight: normal;text=
-decoration: underline;">P.A.C.E. CE&nbsp;=EF=BD=9C&nbsp;Florida CE</a>

<p style=3D"text-align: left;margin: 10px 0;padding: 0;mso-line-height-rul=
e: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;color=
: #202020;font-family: Helvetica;font-size: 14px;line-height: 150%;"><span=
 style=3D"color:#E71216"><strong><span style=3D"font-size:15px">March 08=
=2C 2023</span></strong></span><br>
<a href=3D"https://labroots.us7.list-manage.com/track/click?u=3D6f39393ef3=
8fb324428ff8c2c&id=3D2d8116c530&e=3D6670ee6f6b" target=3D"_blank" style=
=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text-=
size-adjust: 100%;color: #1e8ae7;font-weight: normal;text-decoration: unde=
rline;"><img data-file-id=3D"42943" height=3D"13" src=3D"https://gallery.m=
ailchimp.com/6f39393ef38fb324428ff8c2c/images/b9d0b3b5-66ac-4b86-857f-08e5=
d0e67a46.png" style=3D"border: 0px;width: 20px;height: 13px;margin: 0px;ou=
tline: none;text-decoration: none;-ms-interpolation-mode: bicubic;" width=
=3D"20"></a><span style=3D"font-size:13px">8:00am (PST)=2C 11:00am (EST)=
=2C<br>
&nbsp; &nbsp; &nbsp;5:00pm (CET)</span></p>

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=09=09=09=09

=09=09=09=09
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lpadding=3D"0" cellspacing=3D"0" width=3D"300" class=3D"columnWrapper" sty=
le=3D"border-collapse: collapse;mso-table-lspace: 0pt;mso-table-rspace: 0p=
t;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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                                            <td valign=3D"top" class=3D"co=
lumnContainer" style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust=
: 100%;-webkit-text-size-adjust: 100%;"><table border=3D"0" cellpadding=3D=
"0" cellspacing=3D"0" width=3D"100%" class=3D"mcnTextBlock" style=3D"min-w=
idth: 100%;border-collapse: collapse;mso-table-lspace: 0pt;mso-table-rspac=
e: 0pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
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<strong>Presented by:</strong>
                        </td>
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=09=09=09=09

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</table><table border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" class=3D"mcnTextBlock" style=3D"min-width: 100%;border-collapse: coll=
apse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 100=
%;-webkit-text-size-adjust: 100%;">
    <tbody class=3D"mcnTextBlockOuter">
        <tr>
            <td valign=3D"top" class=3D"mcnTextBlockInner" style=3D"paddin=
g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
                    <tbody><tr>

<span style=3D"color:#E71216"><span style=3D"f=
ont-size:13px"><strong>Kevin Pridham</strong></span></span><br>
<span style=3D"font-size:12px">Field Applications Scientist=2C<br>
Thermo Fisher Scientific</span>
                        </td>
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=09=09=09=09

=09=09=09=09
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                                <td valign=3D"top" id=3D"templateFooter" s=
tyle=3D"background:#ffffff none no-repeat center/cover;mso-line-height-rul=
e: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;backg=
round-color: #ffffff;background-image: none;background-repeat: no-repeat;b=
ackground-position: center;background-size: cover;border-top: 0;border-bot=
tom: 0;padding-top: 9px;padding-bottom: 9px;"><table border=3D"0" cellpadd=
ing=3D"0" cellspacing=3D"0" width=3D"100%" class=3D"mcnTextBlock" style=3D=
"min-width: 100%;border-collapse: collapse;mso-table-lspace: 0pt;mso-table=
-rspace: 0pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
    <tbody class=3D"mcnTextBlockOuter">
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            <td valign=3D"top" class=3D"mcnTextBlockInner" style=3D"paddin=
g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
                    <tbody><tr>

<p style=3D"font-size: 14px;text-align: left;m=
argin: 10px 0;padding: 0;mso-line-height-rule: exactly;-ms-text-size-adjus=
t: 100%;-webkit-text-size-adjust: 100%;color: #555555;font-family: Helveti=
ca;line-height: 150%;"><strong>Abstract</strong><br>
Engineered nucleases like the CRISPR-Cas9 system provide researchers with=
 powerful tools for the manipulation of the genome to create cell-based di=
sease models=2C animal models=2C and cell therapies for clinical implement=
ation. These editing modalities can achieve high editing efficiencies in a=
 wide range of different cell types and gene loci. However=2C this high ed=
iting efficiency comes at a price: Off target cleavage. Growing concerns f=
or off-target editing and the downstream results of such edits have resear=
chers looking for best practices in their genome editing experiments. In t=
his presentation=2C we will explore strategies to limit off-target edits i=
n your gene editing experiments=2C share considerations to take in differe=
nt experimental scenarios=2C and demonstrate the efforts of Thermo Fisher=
 Scientific to optimize the gene editing workflow - simplifying it for all=
 researchers.<br>
&nbsp;<br>
<strong>Learning Objectives</strong></p>

<ul>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;">Summarize the impact of off-target edits i=
n experiments</li>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;">Discuss the methods to detect=2C identify=
=2C and limit off-target edits in your gene editing experiments</li>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;">Assess solutions to streamline and improve=
 the gene editing workflow</li>
</ul>

</td>
                    </tr>
                </tbody></table>
=09=09=09=09

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it-text-size-adjust: 100%;">
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reContentItemContainer">
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g-bottom: 5px;padding-left: 9px;mso-line-height-rule: exactly;-ms-text-siz=
e-adjust: 100%;-webkit-text-size-adjust: 100%;">
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gn=3D"left" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"" st=
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pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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gn=3D"left" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"" st=
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erpolation-mode: bicubic;" height=3D"24" width=3D"24" class=3D""></a>
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td>
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d align=3D"left" valign=3D"middle" class=3D"mcnShareTextContent" style=3D"=
padding-left: 5px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%=
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le: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">Sh=
are</a>
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td>
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table>
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00%" class=3D"mcnTextBlock" style=3D"min-width: 100%;border-collapse: coll=
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pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
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Container">
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<p style=3D"font-size: 14px;line-height: 125%;=
text-align: center;margin: 10px 0;padding: 0;mso-line-height-rule: exactly=
;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;color: #555555;=
font-family: Helvetica;"><span style=3D"font-size:11px"><strong>Can't atte=
nd live?</strong> <a href=3D"https://labroots.us7.list-manage.com/track/cl=
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a><a href=3D"https://labroots.us7.list-manage.com/track/click?u=3D6f39393e=
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t-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;=
color: #1e8ae7;font-weight: normal;text-decoration: underline;"><span styl=
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rded webcast.<br>
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=09=09=09=09

=09=09=09=09
            </td>
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</table><table border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
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            </td>
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</table><table border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" class=3D"mcnDividerBlock" style=3D"min-width: 100%;background-color:=
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ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">&nbsp;</td>   =
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                                          </tr>
                                       </tbody>
                                    </table>
                                             =
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