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Subject: =?utf-8?Q?Webinar=3A=20A=20spatially=2Dresolved=20transcriptional=20atlas=20of=20the=20murine=20dorsal=20pontine=20tegmentum?=
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Date: Thu, 26 Jan 2023 21:30:39 +0000
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Free Webinar - Register Today!

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** A spatially-resolved transcriptional atlas of the murine dorsal pontine=
 tegmentum
------------------------------------------------------------
C.E. Credits:  (https://www.labroots.com/ms/webinar/visualizing-situ-singl=
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February 14=2C 2023
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7:00pm (CET)

Presented by:
https://www.labroots.com/ms/webinar/spatially-resolved-transcriptional-atl=
as-murine-dorsal-pontine-tegmentum?campaign=3Dlremail
Stefano Nardone=2C PhD
Research scientist=2C Harvard Medical School Beth Israel Deaconess Medical=
 Center Center for Life Sciences

=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
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Abstract:
The dorsal pontine tegmentum (PnTg) is part of the Pons=2C the largest sec=
tion of the brainstem. It is a complex region involved in the regulation o=
f many vital functions. However=2C the transcriptome and the spatial organ=
ization of its cells have never been described.

We applied single-nucleus RNA-seq (snRNA-seq) to resolve neuronal subtypes=
 based on their unique transcriptional profile and then multiplexed error=
 robust fluorescence in situ hybridization (MERFISH) to map them spatially=
=2E

Interpreting and gaining insights from spatial data can be a daunting proc=
ess. Static plots are useful for displaying the complexity but cannot quer=
y data in real time.
In collaboration with HEAVY.AI=2C we developed a dashboard that=2C by harn=
essing the massive parallelism of modern GPU and CPU hardware=2C produces=
 interactive visualization of spatial-transcriptomic data that can be quer=
ied analyzed in real-time within the dashboard itself. This interaction ca=
n help uncover insights and identify trends faster=2C leading to better=2C=
 data-driven decisions.

Learning Objectives
* Resolve neuronal subtypes in the murine dorsal pontine tegmentum (PnTg)=
 based on their unique transcriptional profile with snRNA-seq.
* Investigate the spatial organization of the murine PnTg with MERFISH dat=
a.
* Evaluate the use of HEAVY.AI to interpret MERFISH spatial data.

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              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
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<h1 style=3D"display: block;margin: 0;padding:=
 0;color: #333333;font-family: Helvetica;font-size: 24px;font-style: norma=
l;font-weight: normal;line-height: 125%;letter-spacing: normal;text-align:=
 left;">A spatially-resolved transcriptional atlas of the murine dorsal po=
ntine tegmentum</h1>
<br>
<span style=3D"font-size:14px">C.E. Credits:<a href=3D"https://labroots.us=
7.list-manage.com/track/click?u=3D6f39393ef38fb324428ff8c2c&id=3Dccb6ae3b2=
e&e=3D6670ee6f6b" target=3D"_blank" style=3D"mso-line-height-rule: exactl=
y;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;color: #1e8ae7=
;font-weight: normal;text-decoration: underline;">&nbsp;</a><a href=3D"htt=
ps://labroots.us7.list-manage.com/track/click?u=3D6f39393ef38fb324428ff8c2=
c&id=3D8e7b87ad05&e=3D6670ee6f6b" target=3D"_blank" style=3D"mso-line-hei=
ght-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100=
%;color: #1e8ae7;font-weight: normal;text-decoration: underline;"><span st=
yle=3D"color:#E02873">P.A.C.E. CE&nbsp;=EF=BD=9C&nbsp;Florida CE</span></a=
></span>

<p style=3D"text-align: left;margin: 10px 0;padding: 0;mso-line-height-rul=
e: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;color=
: #202020;font-family: Helvetica;font-size: 14px;line-height: 150%;"><span=
 style=3D"color:#E02873"><span style=3D"font-size:14px"><strong>February 1=
4=2C 2023</strong></span></span><br>
<u><a href=3D"https://labroots.us7.list-manage.com/track/click?u=3D6f39393=
ef38fb324428ff8c2c&id=3D7b29bad792&e=3D6670ee6f6b" target=3D"_blank" styl=
e=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text=
-size-adjust: 100%;color: #1e8ae7;font-weight: normal;text-decoration: und=
erline;"><img data-file-id=3D"42943" height=3D"13" src=3D"https://gallery.=
mailchimp.com/6f39393ef38fb324428ff8c2c/images/b9d0b3b5-66ac-4b86-857f-08e=
5d0e67a46.png" style=3D"border: 0px;width: 20px;height: 13px;margin: 0px;o=
utline: none;text-decoration: none;-ms-interpolation-mode: bicubic;" width=
=3D"20"></a></u>10:00am (PST)=2C 1:00pm (EST)=2C<br>
&nbsp; &nbsp; &nbsp;7:00pm (CET)</p>

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=09=09=09=09

=09=09=09=09
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le=3D"border-collapse: collapse;mso-table-lspace: 0pt;mso-table-rspace: 0p=
t;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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lumnContainer" style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust=
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"0" cellspacing=3D"0" width=3D"100%" class=3D"mcnTextBlock" style=3D"min-w=
idth: 100%;border-collapse: collapse;mso-table-lspace: 0pt;mso-table-rspac=
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              =09
=09=09=09
=09=09=09=09
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<br>
<br>
<strong>Presented by:</strong>
                        </td>
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=09=09=09=09

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</table><table border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" class=3D"mcnTextBlock" style=3D"min-width: 100%;border-collapse: coll=
apse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 100=
%;-webkit-text-size-adjust: 100%;">
    <tbody class=3D"mcnTextBlockOuter">
        <tr>
            <td valign=3D"top" class=3D"mcnTextBlockInner" style=3D"paddin=
g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
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</td>
                    </tr>
                </tbody></table>
=09=09=09=09

=09=09=09=09
            </td>
        </tr>
    </tbody>
</table></td>
                                        </tr>
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                                </td>
                            </tr>
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tyle=3D"background:#ffffff none no-repeat center/cover;mso-line-height-rul=
e: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;backg=
round-color: #ffffff;background-image: none;background-repeat: no-repeat;b=
ackground-position: center;background-size: cover;border-top: 0;border-bot=
tom: 0;padding-top: 9px;padding-bottom: 9px;"><table border=3D"0" cellpadd=
ing=3D"0" cellspacing=3D"0" width=3D"100%" class=3D"mcnTextBlock" style=3D=
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            <td valign=3D"top" class=3D"mcnTextBlockInner" style=3D"paddin=
g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
                    <tbody><tr>

<p style=3D"font-size: 14px;text-align: left;m=
argin: 10px 0;padding: 0;mso-line-height-rule: exactly;-ms-text-size-adjus=
t: 100%;-webkit-text-size-adjust: 100%;color: #555555;font-family: Helveti=
ca;line-height: 150%;"><span style=3D"font-family:arial=2Chelvetica neue=
=2Chelvetica=2Csans-serif"><span style=3D"font-size:14px"><strong>Abstract=
:</strong></span></span></p>

<ul>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;"><span style=3D"font-size:14px">Resolve neu=
ronal subtypes in the murine dorsal pontine tegmentum (PnTg) based on thei=
r unique transcriptional profile with snRNA-seq.</span></li>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;"><span style=3D"font-size:14px">Investigate=
 the spatial organization of the murine PnTg with MERFISH data.</span></li=
>
=09<li style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-=
webkit-text-size-adjust: 100%;"><span style=3D"font-size:14px">Evaluate th=
e use of HEAVY.AI to interpret MERFISH spatial data.</span></li>
</ul>
</div>

</td>
                    </tr>
                </tbody></table>
=09=09=09=09

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o-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 100%;-webk=
it-text-size-adjust: 100%;">
                                            <tbody><tr>
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=3D"padding-right: 9px;padding-bottom: 9px;mso-line-height-rule: exactly;-=
ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;" class=3D"mcnSha=
reContentItemContainer">
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eft" valign=3D"middle" style=3D"padding-top: 5px;padding-right: 9px;paddin=
g-bottom: 5px;padding-left: 9px;mso-line-height-rule: exactly;-ms-text-siz=
e-adjust: 100%;-webkit-text-size-adjust: 100%;">
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gn=3D"left" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"" st=
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pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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                                             =
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