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** EM ICE - Workflows in Every Direction
------------------------------------------------------------

November 17=2C 2022
https://www.labroots.com/intercall/add-to-calendar/Webinar/7760?start-date=
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Presented by:
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Seth Villarreal

Advanced Workflow Specialist=2C Nanotechnology=2C Leica Microsystems

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Abstract:
High pressure freezing offers versatile and deep capability in the study o=
f intricate changes in fine structure or cellular dynamics. From the inter=
nals of a single cell to complex layers within small whole organisms=2C th=
e EM ICE occupies a key point in many electron microscopy (EM) workflows.=
 These workflows extend from correlative light and electron microscopy (CL=
EM) methods to straightforward freeze substitution by resin embedding for=
 ultramicrotomy.

This webinar is an overview of the various workflows enabled by high press=
ure freezing on the EM ICE.
- Live cell CLEM for stabilizing transient and rare biological processes
- In situ light and electrical stimulation of live cells=2C with milliseco=
nd timing precision relative to cryofixation
- Freeze substitution for room temperature EM
- Freeze fracture to expose the internal structure of cells
- Lamella milling by focused ion beam
- Cryo ultramicrotomy=2C for cryo transmission EM of thin sections

The EM ICE=2C combining superior high pressure freezing with support for a=
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              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
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<h1 style=3D"display: block;margin: 0;padding:=
 0;color: #333333;font-family: Helvetica;font-size: 24px;font-style: norma=
l;font-weight: normal;line-height: 125%;letter-spacing: normal;text-align:=
 left;"><span style=3D"font-size:24px">EM ICE - Workflows in Every Directi=
on</span></h1>
&nbsp;

<p style=3D"text-align: left;margin: 10px 0;padding: 0;mso-line-height-rul=
e: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;color=
: #202020;font-family: Helvetica;font-size: 14px;line-height: 150%;"><span=
 style=3D"font-size:15px"><strong>November 17=2C 2022</strong></span><br>
<a href=3D"https://labroots.us7.list-manage.com/track/click?u=3D6f39393ef3=
8fb324428ff8c2c&id=3D242c072502&e=3D175eb93a1a" target=3D"_blank" style=
=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text-=
size-adjust: 100%;color: #1e8ae7;font-weight: normal;text-decoration: unde=
rline;"><img data-file-id=3D"42943" height=3D"13" src=3D"https://gallery.m=
ailchimp.com/6f39393ef38fb324428ff8c2c/images/b9d0b3b5-66ac-4b86-857f-08e5=
d0e67a46.png" style=3D"border: 0px;width: 20px;height: 13px;margin: 0px;ou=
tline: none;text-decoration: none;-ms-interpolation-mode: bicubic;" width=
=3D"20"></a><span style=3D"font-size:14px">11:00am (PST)=2C&nbsp; 2:00pm (=
EST)=2C<br>
&nbsp; &nbsp; &nbsp;8:00pm (CEST)</span></p>

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=09=09=09=09

=09=09=09=09
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lpadding=3D"0" cellspacing=3D"0" width=3D"300" class=3D"columnWrapper" sty=
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t;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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lumnContainer" style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust=
: 100%;-webkit-text-size-adjust: 100%;"><table border=3D"0" cellpadding=3D=
"0" cellspacing=3D"0" width=3D"100%" class=3D"mcnTextBlock" style=3D"min-w=
idth: 100%;border-collapse: collapse;mso-table-lspace: 0pt;mso-table-rspac=
e: 0pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
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<strong>Presented by:</strong>
                        </td>
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=09=09=09=09

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00%" class=3D"mcnTextBlock" style=3D"min-width: 100%;border-collapse: coll=
apse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 100=
%;-webkit-text-size-adjust: 100%;">
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            <td valign=3D"top" class=3D"mcnTextBlockInner" style=3D"paddin=
g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
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</td>
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=09=09=09=09

=09=09=09=09
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</table></td>
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tyle=3D"background:#ffffff none no-repeat center/cover;mso-line-height-rul=
e: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;backg=
round-color: #ffffff;background-image: none;background-repeat: no-repeat;b=
ackground-position: center;background-size: cover;border-top: 0;border-bot=
tom: 0;padding-top: 9px;padding-bottom: 9px;"><table border=3D"0" cellpadd=
ing=3D"0" cellspacing=3D"0" width=3D"100%" class=3D"mcnTextBlock" style=3D=
"min-width: 100%;border-collapse: collapse;mso-table-lspace: 0pt;mso-table=
-rspace: 0pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
    <tbody class=3D"mcnTextBlockOuter">
        <tr>
            <td valign=3D"top" class=3D"mcnTextBlockInner" style=3D"paddin=
g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
                    <tbody><tr>

<p style=3D"font-size: 14px;text-align: left;m=
argin: 10px 0;padding: 0;mso-line-height-rule: exactly;-ms-text-size-adjus=
t: 100%;-webkit-text-size-adjust: 100%;color: #555555;font-family: Helveti=
ca;line-height: 150%;"><span style=3D"font-family:arial=2Chelvetica neue=
=2Chelvetica=2Csans-serif"><span style=3D"font-size:14px"><strong>Abstract=
:</strong></span></span></p>
<span style=3D"font-family:arial=2Chelvetica neue=2Chelvetica=2Csans-serif=
"><span style=3D"font-size:14px">High pressure freezing offers versatile a=
nd deep capability in the study of intricate changes in fine structure or=
 cellular dynamics. From the internals of a single cell to complex layers=
 within small whole organisms=2C the EM ICE occupies a key point in many e=
lectron microscopy (EM) workflows. These workflows extend from correlative=
 light and electron microscopy (CLEM) methods to straightforward freeze su=
bstitution by resin embedding for ultramicrotomy.<br>
<strong>&nbsp;<br>
This webinar is an overview of the various workflows enabled by high press=
ure freezing on the EM ICE.</strong><br>
- Live cell CLEM for stabilizing transient and rare biological processes<b=
r>
- In situ light and electrical stimulation of live cells=2C with milliseco=
nd timing precision relative to cryofixation<br>
- Freeze substitution for room temperature EM<br>
- Freeze fracture to expose the internal structure of cells<br>
- Lamella milling by focused ion beam<br>
- Cryo ultramicrotomy=2C for cryo transmission EM of thin sections<br>
&nbsp;<br>
The EM ICE=2C combining superior high pressure freezing with support for a=
 wide variety of workflows=2C is an excellent platform for more than just=
 single project.</span></span>
                        </td>
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                </tbody></table>
=09=09=09=09

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it-text-size-adjust: 100%;">
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g-bottom: 5px;padding-left: 9px;mso-line-height-rule: exactly;-ms-text-siz=
e-adjust: 100%;-webkit-text-size-adjust: 100%;">
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gn=3D"left" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"" st=
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