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Subject: =?utf-8?Q?Reminder=3A=20Efficient=20culturing=20of=20human=20mesenchymal=20stromal=20cells=20for=20cell=20therapy=20research?=
From: =?utf-8?Q?Labroots=20for=20Thermo=20Fisher=20Scientific?= <info@labroots.com>
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To: <kibrahim@saraya.world>
Date: Tue, 18 Oct 2022 18:03:33 +0000
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Free Webinar - Register Today!

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-based-protocol-to-detect-and-quantify-rna-editing-events-at-hotspots-2267=
50?e=3D24e951eba2)
https://www.labroots.com/ms/webinar/efficient-culturing-human-mesenchymal-=
stromal-cells-cell-therapy-research?campaign=3Dlremai
Register for Free (https://www.labroots.com/ms/webinar/efficient-culturing=
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** Efficient culturing of human mesenchymal stromal cells for cell therapy=
 research
------------------------------------------------------------

October 19=2C 2022
https://www.labroots.com/intercall/add-to-calendar/Webinar/7691?start-date=
=3D20221019T090000&end-date=3D20221019T100000&TZID=3DAmerica/Los_Angeles9:=
00am (PDT)=2C  12:00pm (EDT)=2C
6:00pm (CEST)
Presented by:
https://www.labroots.com/ms/webinar/efficient-culturing-human-mesenchymal-=
stromal-cells-cell-therapy-research?campaign=3Dlremail?campaign=3Dlremail
Kanika Singh
Research and Development Scientist -
Thermo Fisher Scientific

=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
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=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D

Abstract
Stem cell therapy=2C in which stem cells are used for disease treatment or=
 tissue repair=2C is an increasingly active area of research. Treatment of=
 many chronic diseases with mesenchymal stromal cells (MSCs) has yielded p=
romising results for inducing tissue repair and regeneration. Human mesenc=
hymal stromal cells (hMSCs) are widely used in cell therapy because of the=
ir ease of harvest=2C self-renewal=2C and ability to suppress activation o=
f innate and adaptive immune response. Furthermore=2C hMSCs release bioact=
ive molecules and possess homing properties which are important for tissue=
 repair. These distinct properties of hMSCs result in progression of treat=
ments for a wide range of diseases.
For culturing and expansion of hMSCs in vitro; fetal bovine serum (FBS) is=
 routinely used=2C but it carries the risk of xeno-immunity & zoonotic tra=
nsmission if such cells are to be used for cell therapy. For this reason=
=2C chemically defined xeno-free and serum-free media are used to culture=
 therapeutic stem cells for translational research. However=2C culturing w=
ith serum-free media requires coating of the cell culture surface with ext=
racellular matrix (ECM) proteins. These proteins can trigger an uncontroll=
ed immune response and affect graft integration. To use hMSCs for cell the=
rapy research=2C it is important to avoid these immune response eliciting=
 substances. For efficient invitro culturing of hMSCs without the need of=
 serum or ECM coating=2C Nunclon Supra is one of the specialized high atta=
chment surfaces available in the market. Growing cells on such surfaces ca=
n help with more consistent results and smooth transition into clinical ap=
plications while maintaining high cell quality and
potency.

The efficacy of cell therapy is not only based on the quality and phenotyp=
e of cells=2C but also on transplantation methodology. Traditionally=2C tr=
ansplantation of MSCs required the use of biological scaffolds=2C whose de=
gradation would lead to fibrosis. Thus=2C researchers these days are using=
 scaffold-free cell sheet technique which enables viable cell grafting at=
 host site. Contrary to the traditional cell injections where cells disapp=
ear in a short time; cells from a sheet are targeted on the damaged area=
=2C and multiple sheets could also be transplanted. Fabrication of hMSC sh=
eets is becoming an important tool for regenerative medicine. These sheets=
 can be generated in a scaffold free format by using temperature-responsiv=
e surfaces. Nunc Upcell is one such surface which permits cell harvest wit=
hout cell protein damage. Harvested sheet preserves ECMs=2C cell=E2=80=93c=
ell junctions=2C and cell=E2=80=93ECM junctions which are important for ef=
fective cell-based therapy. In summary=2C the use of hMSCs in translationa=
l
research=2C is accelerating. hMSCs exhibit distinct advantages in cell the=
rapy=2C and it is important to provide precise culture conditions for thei=
r growth and proliferation. The experimental data presented here demonstra=
te the application of Nunclon Supra surface to facilitate GMP culturing of=
 hMSCs and highlights the application of Nunc UpCell surface to fabricate=
 hMSCs cell sheets. Overall=2C this would help MSCs translation to clinica=
l practice while maintaining their safety=2C quality=2C and efficacy stand=
ards.

Learning Objectives
* Basics of cell therapy and MSCs
* hMSCs expansion=2C characterization=2C and differentiation under serum-f=
ree=2C xeno-free and coating free conditions
* Fabrication of hMSC sheets

** Register now (https://www.labroots.com/ms/webinar/efficient-culturing-h=
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              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
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<h1 style=3D"display: block;margin: 0;padding:=
 0;color: #333333;font-family: Helvetica;font-size: 24px;font-style: norma=
l;font-weight: normal;line-height: 125%;letter-spacing: normal;text-align:=
 left;">Efficient culturing of human mesenchymal stromal cells for cell th=
erapy research</h1>

<p style=3D"text-align: left;margin: 10px 0;padding: 0;mso-line-height-rul=
e: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;color=
: #202020;font-family: Helvetica;font-size: 14px;line-height: 150%;"><span=
 style=3D"color:#E71216"><strong>October&nbsp;19=2C 2022</strong></span><b=
r>
<a href=3D"https://labroots.us7.list-manage.com/track/click?u=3D6f39393ef3=
8fb324428ff8c2c&id=3D3460e474d8&e=3D24e951eba2" target=3D"_blank" style=
=3D"mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webkit-text-=
size-adjust: 100%;color: #1e8ae7;font-weight: normal;text-decoration: unde=
rline;"><img data-file-id=3D"42943" height=3D"13" src=3D"https://gallery.m=
ailchimp.com/6f39393ef38fb324428ff8c2c/images/b9d0b3b5-66ac-4b86-857f-08e5=
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tline: none;text-decoration: none;-ms-interpolation-mode: bicubic;" width=
=3D"20"></a><span style=3D"font-size:13px">9:00am (PDT)=2C&nbsp; 12:00pm (=
EDT)=2C<br>
&nbsp; &nbsp; &nbsp; 6:00pm (CEST)</span></p>

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=09=09=09=09

=09=09=09=09
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lpadding=3D"0" cellspacing=3D"0" width=3D"300" class=3D"columnWrapper" sty=
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t;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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lumnContainer" style=3D"mso-line-height-rule: exactly;-ms-text-size-adjust=
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"0" cellspacing=3D"0" width=3D"100%" class=3D"mcnTextBlock" style=3D"min-w=
idth: 100%;border-collapse: collapse;mso-table-lspace: 0pt;mso-table-rspac=
e: 0pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
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<strong>Presented by:</strong>
                        </td>
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=09=09=09=09

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00%" class=3D"mcnTextBlock" style=3D"min-width: 100%;border-collapse: coll=
apse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 100=
%;-webkit-text-size-adjust: 100%;">
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g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
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<span style=3D"color:#E71216"><span style=3D"f=
ont-size:13px"><strong>Kanika Singh</strong></span></span><br>
Research and Development Scientist -<br>
Thermo Fisher Scientific
                        </td>
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=09=09=09=09

=09=09=09=09
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tyle=3D"background:#ffffff none no-repeat center/cover;mso-line-height-rul=
e: exactly;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;backg=
round-color: #ffffff;background-image: none;background-repeat: no-repeat;b=
ackground-position: center;background-size: cover;border-top: 0;border-bot=
tom: 0;padding-top: 9px;padding-bottom: 9px;"><table border=3D"0" cellpadd=
ing=3D"0" cellspacing=3D"0" width=3D"100%" class=3D"mcnTextBlock" style=3D=
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            <td valign=3D"top" class=3D"mcnTextBlockInner" style=3D"paddin=
g-top: 9px;mso-line-height-rule: exactly;-ms-text-size-adjust: 100%;-webki=
t-text-size-adjust: 100%;">
              =09
=09=09=09
=09=09=09=09
                <table align=3D"left" border=3D"0" cellpadding=3D"0" cells=
pacing=3D"0" style=3D"max-width: 100%;min-width: 100%;border-collapse: col=
lapse;mso-table-lspace: 0pt;mso-table-rspace: 0pt;-ms-text-size-adjust: 10=
0%;-webkit-text-size-adjust: 100%;" width=3D"100%" class=3D"mcnTextContent=
Container">
                    <tbody><tr>

<p style=3D"font-size: 14px;text-align: left;m=
argin: 10px 0;padding: 0;mso-line-height-rule: exactly;-ms-text-size-adjus=
t: 100%;-webkit-text-size-adjust: 100%;color: #555555;font-family: Helveti=
ca;line-height: 150%;"><span style=3D"font-size:14px"><span style=3D"font-=
family:arial=2Chelvetica neue=2Chelvetica=2Csans-serif"><strong>Abstract</=
strong></span></span></p>

<ul>
=09<li style=3D"font-size: 14px;mso-line-height-rule: exactly;-ms-text-siz=
e-adjust: 100%;-webkit-text-size-adjust: 100%;">Basics of cell therapy and=
 MSCs</li>
=09<li style=3D"font-size: 14px;mso-line-height-rule: exactly;-ms-text-siz=
e-adjust: 100%;-webkit-text-size-adjust: 100%;"><span style=3D"font-family=
:arial=2Chelvetica neue=2Chelvetica=2Csans-serif">hMSCs expansion=2C chara=
cterization=2C and differentiation under serum-free=2C xeno-free and coati=
ng free conditions</span></li>
=09<li style=3D"font-size: 14px;mso-line-height-rule: exactly;-ms-text-siz=
e-adjust: 100%;-webkit-text-size-adjust: 100%;"><span style=3D"font-family=
:arial=2Chelvetica neue=2Chelvetica=2Csans-serif">Fabrication of hMSC shee=
ts</span></li>
</ul>

</td>
                    </tr>
                </tbody></table>
=09=09=09=09

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it-text-size-adjust: 100%;">
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=3D"padding-right: 9px;padding-bottom: 9px;mso-line-height-rule: exactly;-=
ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;" class=3D"mcnSha=
reContentItemContainer">
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llpadding=3D"0" cellspacing=3D"0" width=3D"" class=3D"mcnShareContentItem"=
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g-bottom: 5px;padding-left: 9px;mso-line-height-rule: exactly;-ms-text-siz=
e-adjust: 100%;-webkit-text-size-adjust: 100%;">
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gn=3D"left" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"" st=
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pt;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%;">
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                                             =
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